ࡱ > W Y J K L M N O P Q R S T U V f y y q` R DM bjbjqPqP : : iD t t ; ; ; H > @ t A @ E E E E .I .I .I $ h j V H ^ I " V V E E f f f V E E f V f f qs | t E A p!WME ; b , s 0 s e Z $ t u w D .I ` K f @M \ N ; .I .I .I sf X .I .I .I V V V V t t t D3 ; t t t ; t t t ezS1ukXQ D O I : 1ukXQ NTkXe[uirn`l_uirv;m'`vq_T q1 , 2 , 3 FQ1 , 2 , 3 !P[g_1 , 2 , 3 m1 , 2 , 3 1 . TNm'Yf[ sXyf[N]zf[b Nwm 2 0 0 0 9 2 2 . TNm'Yf[ uirb/gxvz@b Nwm 2 0 0 0 9 2 3 . TNm'Yf[ _l4lsXYe͑p[[ Nwm 2 0 0 0 9 2 Xduirn`l/f Ny(uNRce_QgGal4lallYtvu`Wb/g0kXe/fN_uirVNuX[vN([N_uirutu`\O(uvS%cwQ g͑vq_T0(W g:gwS%cS'``nmir(ϑSm^ :N2 . 1 1 k g ( m - 3 "d - 1 ) vagNN[(gs0ttT\s NykXe[uirn`lYtiRYOall]z'`vq_T0Ǐv;m'`T_uirv;m'`vSSRg NykXe\O:Nuirn`lkXevSL'`0~ghf(W NykXevuirn`lGWn g:gir( V S S ) vQϑs'YN4 0 % vMRcNNttT\svk(gsuirn`lQv'lSirgkSvS O D ;m'` gNOx'`xxvA C P ;m'` gؚ_uirv1"lvI N T - D H A ;m'` gؚhf(W(gskXev_sX-NS0Rv g\N_uirMR gؚVdk(gskXe)RNuirn`l]zHevS%c0 sQ.͋uirn`lؚHekXeT_uirv;m'`utu`^'` -NVR{|SX 7 0 3 . 1 e.sh_xA I m p a c t s o f f i l t e r m e d i a o n e n z y m a t i c a c t i v i t i e s i n e a r t h w o r m b i o f i l t e r s X I N G M e i y a n 1 , 2 , 3 , L U Y u f a n 1 , 2 , 3 , Z H E N G Y a d a n 1 , 2 , 3 , C A I L u z i y u 1 , 2 , 3 , X I A O T a o 1 , 2 , 3 ( 1 . C o l l e g e o f E n v i r o n m e n t a l S c i e n c e a n d E n g i n e e r i n g , T o n g j i U n i v e r s i t y , S h a n g h a i 2 0 0 0 9 2 , C h i n a 2 . I n s t i t u t e o f B i o f i l m T e c h n o l o g y , T o n g j i U n i v e r s i t y , S h a n g h a i 2 0 0 0 9 2 , C h i n a 3 . K e y L a b o r a t o r y o f Y a n g t ze River Water Environment < Ministry of Education > , Shanghai 200092, China) Abstract: Vermifiltration is an ecological technology that applys to decentralized wastewater and excess sludge treatment. Filter media, crucial to earthworms and microbes to habitat, significantly influence physiological and ecological functions of earthworms and microbes. At the organic loading (mass concentration of volatile suspended solids) of 2.11kg/(m3 "d ) , e f f e c t s o f t h e t h r e e f i l t e r m e d i a ( w o o d e n b e a d , p o r c e l a i n b a l l a n d p a l l r i n g ) o n e x c e s s s l u d g e t r e a t m e n t p e r f o r m a n c e w e r e i n v e s t i g a t e d b y e x p l o r i n g t h e v a r i a t i o n s o f e n z y m a t i c a c t i v i t i e s o f e a r t h w o r m s a n d o f m i c r o b e s . T h e r e s u l t s d e m o n s t r a t e d t h a t t h e VSS reduction by three earthworm biofilters enable to meet the requirement of execess sludge treatment (upwards of 40%). Notably, earthworm biofilters filled with wooden bead can offer the best habitat for earthworms and microbes to survive. Specifically, for earthworms, their SOD and ACP behaved the lowest and highest values among the fiter media, respectively, indicating that earthworms sufferred less adverse effect from wooded bead filter material. Furthermore, the highest DHA value of microbes also supported that the wooden bead was the optimum filter material for biochemical degradtaion concideration. logical in physiology and ecology behaved , earthworm enzymatic activities of superoxyde dismutase are lowest and of acid phosphatase are highest and microbe dehydrogenase enzymatic activities are highest. It indicates that and microbes perform best their degradation function. Therefore, wooden bead is good for performance of earthworm biofilters. Key words: earthworm biofilters; efficient filter media; enz y m a t i c a c t i v i t y ; p h y s i o l o g i c a l a n d e c o l o g i c a l a d a p t a t i o n vMRiRYOallVvQ+T4lsؚ NfYtN+T gkir(b:NN_㉳Qv0u`n`lOnc XR%{Q~Q\ϑ;`ϑ vu`ёW[TXSt(Wuirn`l-N_eQ^TibU\NS gv_uirN"W,g[siRYOallv[hQR0RiRYOallvؚHeQϑT3z[SHeg0uirn`lvQϑS;NO`_uirTvOST\O(u0_uirTGWN*bYuvall:NߘeRvQ-Nv g:girTe[NN)RqQu0vNq_T0~ǏvDdߘ0cm0|mRlT|clI{;mR_uirvyTpeϑ'Y'Y9eSfNalgirM[ 1 ] Te[|~-N_uirv b'`DdߘSNndSb{kNv~̃[ 2 ] cؚ_uir;m'`OO=Nt^{S;`SO Nw0R:_SalgirSdvHeg0 _uirT/fuirn`lSdalgirv;NRQ\O:Nu;mRv:W@bkXevk͑0T[sNSkXeNvNT'`I{[utu`\O(uvS%c g@w͑vq_T0TvkXezhꁂY Y'lR:_\O(uS%cck8^&TRS0R NTz^v\O(uq_Tn`lv]z'`0TekXe_N/f_uirD@wuv͑:W@bkXevkhby'Y\hb'`(q_T0R_uirvD@wNeHN"0Vdk,geR+RN(gs0ttT\s:NkXenN N~s^Lvuirn`lňnRek8h]z'`v^NN_uirvv;m'`:NchǏ[S0R도vz^T_uirMRv'Y\e(Wc[TT_uiruX[;mRvkXeTsXagN0 9hncMRg[xvz(W2 . 1 1 k g ( m - 3 "d - 1 ) v g:gwagNNuirn`lW,g0R3z[ЏLv^N(Wdkؚ g:gwN ib'Y NTkXe[uirn`lTychvq_Tz^0(WdkW@x N;NǏ[YLusXv^oz^TDdߘRaϑ[sXv^'`R+R bb'lSvTmSvwQSOxvz0S O D 'lSirgkSv 0C A T Ǐ'lS"lv TP O D Ǐ'lSirv /f Ny gwQNh'`vb'lS2_|~v)R(uv;m'`vSSegxvz[YLu^oz^0A C P x'`xxv TA K P x'`xxv /f$NyxQWvmSvS fv;`SODdߘR0S NebǏ[_uir1"lv;m'`vSSxvz_uir[W(vMRT_uirv;`SO;m'`0 1 PgeNel 1 . 1 Ջ(ul ՋN Nwm^f3al4lS;m'`alllNl`l-NvVAmall\O:NSlvQ'`(h1 0ۏlvS%cS'``nmirv o l a t i l e s u s p e n d e d s o l i d s , V S S N;``nmirs u s p e n d e d s o l i d s , S S v(ϑk