In order to study the coordinated level of the port cluster and urban agglomeration， the evaluation index system of economic development quality of urban agglomeration and development level of port cluster in Yangtze River Delta region is constructed， and a gravity model is used to calculate the radiation capacity of each port in the port cluster to the urban agglomeration. On the basis of gravity model， a coupling degree model and a coupling coordination degree model are used to measure the coordinated development level of the Yangtze River Delta port cluster-urban agglomeration complex system， and calculate the coordinated development level of the port cluster which is used to analyze the influence of port co-opetition strategy on the coordination level of port cluster-urban agglomeration system. The results show that an initial coordinated layout has been formed between the port cluster and the urban agglomeration in Yangtze River Delta region， and it is moving forward to a high level of coordinated development. Besides， the trend that ports choose dislocation competition and active cooperation can enhance the level of integrated and coordinated development of port cluster and promote high-quality integrated development of the Yangtze River Delta.